Original Article

Exp Neurobiol 2009; 18(1): 57-61

Published online June 30, 2009

© The Korean Society for Brain and Neural Sciences

Manganese-Enhanced Magnetic Resonance Imaging of the Spinal Cord in Rats

Myeoung Hoon Cha1, Chulhyun Lee2, Jee Hyun Cho2, Myung-Ae Chung3, Jin-Hun Sohn4, Chaejoon Cheong2, Hye-Jung Lee5 and Bae Hwan Lee1*

1Department of Physiology and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, 2Korea Basic Science Institute, Daejeon 350-333, 3Electronics and Telecommunications Research Institute, Daejeon 305-350, 4Department of Psychology, Chungnam Nat'l University, Daejeon 305-764, 5Acupuncture and Meridian Science Research Center, Kyung Hee University, Seoul 130-701, Korea

Correspondence to: *To whom correspondence should be addressed.
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Manganese-enhanced magnetic resonance imaging (MEMRI) offers a novel neuroimaging method in visualizing the activity patterns of neural circuits. MEMRI is using the divalent manganese ion, which has been used as a cellular contrast agent. The present study was conducted to determine the contrast-enhancing effects of manganese ion administered into the spinal cord of rats. Manganese ion was administered into the spinal cord by lumbar puncture. Ex vivo magnetic resonance images were obtained at 6, 12, 24, and 48 hours after manganese ion injection. Although the highly contrasted images were not observed 6 or 12 hr after manganese injection, the distinctive manganese-enhanced images began to appear at 24 hours after manganese ion injection. These results suggest that the gray matter is the foci of intense paramagnetic signals and MEMRI may provide an effective technique to visualize the activity-dependent patterns in the spinal cord.

Keywords: manganese, magnetic resonance imaging, spinal cord, rat