Exp Neurobiol 2010; 19(1): 49-53
Published online June 30, 2010
© The Korean Society for Brain and Neural Sciences
Hyuna Noh and Soochul Park*
Department of Biological Science, Sookmyung Women's University, Seoul 140-742, Korea
Correspondence to: *To whom correspondence should be addressed.
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EphA/ephrin-A mediated signaling has emerged as a key mechanism regulating axon guidance and topographic mapping, particularly in the well-characterized visual system from the retina to the superior colliculus (SC). In this study, EphA8 bacterial artificial chromosome (BAC) was manipulated to contain a floxed eGFP and human ephrin-A5 expression cassette using homologous recombination method. In the mice containing the recombinant BAC, it was shown that GFP is expressed in an anterior>posterior gradient in the SC. Furthermore, when these mice were crossed with the transgenic mice expressing Cre under the EphA8 promoter, it was evident that a GFP expression cassette was eliminated, and that human ephrin-A5 was ectopically expressed in the anterior region of the SC. This transgenic model would be useful to analyze the role of ephrin-A5 in the SC during the retinocollicular topography formation.
Keywords: ephrin-A5, EphA8, superior colliculus, topographic mapping