Articles

Article

Original Research

Exp Neurobiol 2010; 19(1): 49-53

Published online June 30, 2010

https://doi.org/10.5607/en.2010.19.1.49

© The Korean Society for Brain and Neural Sciences

Ectopic Expression of Ephrin-A5 Under the EphA8 Promoter at the Anterior Region of the Superior Colliculus

Hyuna Noh and Soochul Park*

Department of Biological Science, Sookmyung Women's University, Seoul 140-742, Korea

Correspondence to: *To whom correspondence should be addressed.
TEL: 82-2-710-9330, FAX: 82-2-715-9331
e-mail: scpark@sookmyung.ac.kr

Abstract

EphA/ephrin-A mediated signaling has emerged as a key mechanism regulating axon guidance and topographic mapping, particularly in the well-characterized visual system from the retina to the superior colliculus (SC). In this study, EphA8 bacterial artificial chromosome (BAC) was manipulated to contain a floxed eGFP and human ephrin-A5 expression cassette using homologous recombination method. In the mice containing the recombinant BAC, it was shown that GFP is expressed in an anterior>posterior gradient in the SC. Furthermore, when these mice were crossed with the transgenic mice expressing Cre under the EphA8 promoter, it was evident that a GFP expression cassette was eliminated, and that human ephrin-A5 was ectopically expressed in the anterior region of the SC. This transgenic model would be useful to analyze the role of ephrin-A5 in the SC during the retinocollicular topography formation.

Keywords: ephrin-A5, EphA8, superior colliculus, topographic mapping