Articles

Article

Original Article

Exp Neurobiol 2010; 19(2): 71-74

Published online September 30, 2010

https://doi.org/10.5607/en.2010.19.2.71

© The Korean Society for Brain and Neural Sciences

Isolation and Characterization of Chicken NPAS3

Jiheon Shin, Hye Yun Jeong, Kyung Eun Lee and Jaesang Kim*

Division of Life and Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, Korea

Correspondence to: *To whom correspondence should be addressed.
TEL: 82-2-3277-3414, FAX: 82-2-3277-3760
e-mail: jkim1964@ewha.ac.kr

Abstract

Here we describe characterization of chicken neuronal Per-Arnt-Sim domain 3 (NPAS3) gene during embryogenesis including examinations of expression pattern and function of the gene. RTPCR assay showed that the primary tissue of expression for this gene is the central nervous system (CNS) while RNA in situ hybridization assay confirmed that NPAS3 was expressed in the ventricular zone of developing neural tube as early as Hamburger-Hamilton (HH) stage 20. Ectopic over-expression of the gene in ovo in the developing chicken neural tube by electroporation had little effect on stem cell population, overall neurogenesis, and motor neuron differentiation. We discuss the implications of our observation.

Keywords: NPAS3, CNS, neural tube, chicken, in ovo electroporation