xCITED2 Induces Neural Genes in Animal Cap Explants of Xenopus Embryos
Jaeho Yoon1,#, Jung-Ho Kim1,#, Ok-Joo Lee2, Saet-Byeol Yu3, Jong-Il Kim3, Sung Chan Kim1, Jae-Bong Park1, Jae-Yong Lee1 and Jaebong Kim1*
1Department of Biochemistry, College of Medicine, 2Facial Skeleton Bio Institute, Hallym University, Chuncheon 200-702, 3Genomic Medicine Institute (GMI), Medical Research Center, Seoul National University, Seoul 110-799, Korea
Correspondence to: #These authors equally contributed to this work.
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Neural tissue is arisen from presumptive ectoderm via inhibition of bone morphogenetic protein (BMP) signaling during Xenopus early development. Previous studies demonstrate that ectopic expression of dominant negative BMP4 receptor (DNBR) produces neural tissue in animal cap explants (AC) and also increases the expression level of various genes involved in neurogenesis. To investigate detail mechanism of neurogenesis in transcriptional level, we analyzed RNAs increased by DNBR using total RNA sequencing analysis and identified several candidate genes. Among them, xCITED2 (Xenopus CBP/p300-interacting transcription activator) was induced 4.6 fold by DNBR and preferentially expressed in neural tissues at tadpole stage. Ectopic expression of xCITED2 induced anterior neural genes without mesoderm induction and reduced BMP downstream genes, an eye specific marker and posterior neural marker. Taken together, these results suggest that xCITED2 may have a role in the differentiation of anterior neural tissue during Xenopus early development.
Keywords: xCITED2, DNBR, neurogenesis, Xenopus