• the Korean Society for Brain and Neural Sciences


Original Article

Exp Neurobiol 2015; 24(3): 206-210

Published online September 30, 2015

© The Korean Society for Brain and Neural Sciences

Membrane-Introduction Mass Spectrometry Analysis of Desflurane, Propofol and Fentanyl in Plasma and Cerebrospinal Fluid for Estimation BBB Properties

Vyacheslav Yu. Cherebillo1, Andrei Yu. Elizarov2* and Andrei V. Polegaev1

1Kirov Medical Academy, St. Petersburg 194 044, 2Ioἀe Physical–Technical Institute, St. Petersburg 194 026, Russia

Correspondence to: *To whom correspondence should be addressed.
TEL: 812 297-2245, FAX: 812 297-1017

Received: May 26, 2015; Revised: July 30, 2015; Accepted: August 2, 2015


A possibility to use the Membrane-Introduction Mass Spectrometry (MIMS) with membrane separator interface has evolved into a powerful method for measurement of anaesthetic agents absolute concentration in blood plasma and cerebrospinal fluid for the study of blood-brain barrier (BBB) properties. Recent advanced a new membrane material was used for drug concentration measurement in biologic fluids. A hydrophobic membrane was used in the interface to separate anaesthetic agents from biological fluids: inhalational anaesthetic desflurane,hypnotic propofol, analgesic fentanyl. The selective detection of volatile anesthetic agents in blood does not require long-term sample processing before injecting the sample into mass-spectrometer interface, in contrast to chromatographic methods. Mass-spectrometric interface for the measurement of anaesthetic agent concentration in biological fluids (blood plasma and cerebrospinal fluid) is described. Sampling of biological fluids was performed during balanced inhalational (desflurane, fentanyl) anaesthesia and total intravenous (propofol, fentanyl) anaesthesia.

Keywords: membrane, mass-spectrometer, anesthesia, desflurane, fentanyl