• the Korean Society for Brain and Neural Sciences


Original Article

Exp Neurobiol 2019; 28(4): 474-484

Published online August 31, 2019

© The Korean Society for Brain and Neural Sciences

Distinct Topographical Patterns of Spike-Wave Discharge in Transgenic and Pharmacologically Induced Absence Seizure Models

Soojung Lee1, Eunjin Hwang2, Mina Lee2,3 and Jee Hyun Choi2,3*

1Department of Oral Physiology, Faculty of Dentistry, Kyung Hee University, Seoul 02447, 2Center for Neuroscience, Korea Institute of Science and Technology, Seoul 02792, 3Department of Neuroscience, University of Science and Technology, Daejeon 34113, Korea

Correspondence to: *To whom correspondence should be addressed.
TEL: 82-2-958-6952, FAX: 82-2-958-6737

Received: May 8, 2019; Revised: June 18, 2019; Accepted: July 29, 2019

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License( which permits unrestricted non-commercial use, distribution, andreproduction in any medium, provided the original work is properly cited.

Absence seizures (AS) are generalized non-convulsive seizures characterized by a brief loss of consciousness and spike-and-wave discharges (SWD) in an electroencephalogram (EEG). A number of animal models have been developed to explain the mechanisms of AS, and thalamo-cortical networks are considered to be involved. However, the cortical foci have not been well described in mouse models of AS. This study aims to use a high density EEG in pathophysiologically different AS models to compare the spatiotemporal patterns of SWDs. We used two AS models: a pharmacologically induced model (gamma-hydroxybutyric acid, GHB model) and a transgenic model (phospholipase beta4 knock-out, PLCβ4 model). The occurrences of SWDs were confirmed by thalamic recordings. The topographical analysis of SWDs showed that the onset and propagation patterns were markedly distinguishable between the two models. In the PLCβ4 model, the foci were located within the somatosensory cortex followed by propagation to the frontal cortex, whereas in the GHB model, a majority of SWDs was initiated in the prefrontal cortex followed by propagation to the posterior cortex. In addition, in the GHB model, foci were also observed in other cortical areas. This observation indicates that different cortical networks are involved in the generation of SWDs across the two models.

Graphical Abstract

Keywords: Electroencephalogram, Mice, Absence seizure, Spike-wave discharge