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  • the Korean Society for Brain and Neural Sciences

Article

Short Communication

Exp Neurobiol 2020; 29(6): 417-424

Published online December 7, 2020

https://doi.org/10.5607/en20050

© The Korean Society for Brain and Neural Sciences

Notch Signaling Controls Oligodendrocyte Regeneration in the Injured Telencephalon of Adult Zebrafish

Hwan-Ki Kim1, Dong-won Lee1, Eunmi Kim1, Inyoung Jeong1, Suhyun Kim1*, Bum-Joon Kim2* and Hae-Chul Park1*

1Department of Biomedical Sciences, College of Medicine, Korea University,
2Department of Neurosurgery, Korea University Ansan Hospital, Ansan 15355, Korea

Correspondence to: *To whom correspondence should be addressed.
Hae-Chul Park, TEL: 82-31-412-6713, FAX: 82-31-412-6729
e-mail: hcpark67@korea.ac.kr
Bum-Joon Kim, TEL: 82-31-412-5050, FAX: 82-31-412-5054
e-mail: nsbjkim@korea.ac.kr
Suhyun Kim, TEL: 82-31-412-6725, FAX: 82-31-412-6729
e-mail: dieslunae@naver.com

Received: October 13, 2020; Revised: November 23, 2020; Accepted: November 24, 2020

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

The myelination of axons in the vertebrate nervous system through oligodendrocytes promotes efficient axonal conduction, which is required for the normal function of neurons. The central nervous system (CNS) can regenerate damaged myelin sheaths through the process of remyelination, but the failure of remyelination causes neurological disorders such as multiple sclerosis. In mammals, parenchymal oligodendrocyte progenitor cells (OPCs) are known to be the principal cell type responsible for remyelination in demyelinating diseases and traumatic injuries to the adult CNS. However, growing evidence suggests that neural stem cells (NSCs) are implicated in remyelination in animal models of demyelination. We have previously shown that olig2+ radial glia (RG) have the potential to function as NSCs to produce oligodendrocytes in adult zebrafish. In this study, we developed a zebrafish model of adult telencephalic injury to investigate cellular and molecular mechanisms underlying the regeneration of oligodendrocytes. Using this model, we showed that telencephalic injury induced the proliferation of olig2+ RG and parenchymal OPCs shortly after injury, which was followed by the regeneration of new oligodendrocytes in the adult zebrafish. We also showed that blocking Notch signaling promoted the proliferation of olig2+ RG and OPCs in the normal and injured telencephalon of adult zebrafish. Taken together, our data suggest that Notch-regulated proliferation of olig2+ RG and parenchymal OPCs is responsible for the regeneration of oligodendrocytes in the injured telencephalon of adult zebrafish.

Graphical Abstract


Keywords: Neural stem cells, Oligodendroglia, Regeneration, Telencephalon, Wounds and injuries, Zebrafish